rapid microbiology website
Click here for more details
NAVIGATE
HOME
News
Events/Meetings
Test Methods
Search
Newsletter
 
SEARCH NEWS
For microbes [a..z]:
A B C D E F G H I
J K L M N 0 P Q R
S T U V W X Y Z  

FIND
Suppliers
A..Z
Product Finder
by Country
Service Labs.
Find a Laboratory
Other
B2B
Links
 
THIS SITE
Add to site
News item
Event/Meeting
Supplier details
Service Lab.
About
Advertising
Content
Privacy
RSS/XML? what is RSS/XML?
News word search
 
SUBSCRIBE TO NEWS

BBL™ CHROMagar™ O157 Medium Receives AOAC™-RI Approval

BBL™ CHROMagar™ O157
For the Rapid Detection of E. coli O157 From Food

Escherichia coli serotype O157:H7 can cause serious illness or death when consumed in contaminated foods. Outbreaks of foodborne illness have been caused by the consumption of undercooked or raw ground beef, dry-cured salami, unpasteurized fruit juices, raw milk, cheese curds, lettuce, alfalfa sprouts and game meat.[1]

Conventional methods to isolate E. coli O157:H7 from food involve pre-enrichment to resuscitate damaged cells, enrichment and inoculation to selective, differential media such as MacConkey Agar with sorbitol, tellurite and cefixime. Most non-O157:H7 E. coli ferment sorbitol. However, atypical strains, along with other sorbitol nonfermenting bacteria such as Morganella and Hafnia, appear identical to O157:H7 colonies on sorbitol media.

BBL™ CHROMagar™ O157 was developed for the isolation and differentiation of E. coli O157:H7 from food, environmental or clinical samples. The medium distinguishes this virulent strain from non-O157 E. coli using a highly specific chromogenic reaction. E. coli O157:H7 produces mauve-colored colonies on BBL™ CHROMagar™ O157. Gram-negative organisms, other than E. coli O157, will either be inhibited or produce colorless, blue, green or blue-green (aqua) colonies.

BBL™ CHROMagar™ O157 is certified by the AOAC&#;153; - Research Institute (AOAC-RI) under the Performance Tested Methods[SM] Program for the analysis of raw ground beef and unpasteurized apple cider when using FDA BAM, USDA FSIS, and ISO methods.[2-4]

Performance Characteristics[5]
  • Sensitivity 100% and specificity 100%
  • No false negatives were found in testing the food matrices
  • Detected more positives than the reference method media
  • Plates read with reportable results 24 hours from sample enrichment
  • 24-hour growth-based reportable results when using enhanced Buffered Peptone Water enrichment protocol and the PATHATRIX™ IMS Purification/Concentration system.[6,7]

Only BBL™ CHROMagar™ Formulations Have AOAC™-RI Approval!

BBL™ CHROMagar™ Family

Cat. No.

Unit

BBL™ CHROMagar™ Listeria
215085
20 plates
BBL™ CHROMagar™ O157
214984
20 plates
BBL™ CHROMagar™ Salmonella
214983
20 plates
BBL™ CHROMagar™ Staph aureus**
214982
20 plates
Associated Products
Cat. No.
Unit
Difco™ Buffered Peptone Water 218105 500 g
Difco™ EC Medium, Modified 234020 500 g
Difco™ TSB, Modified 292735 500 g
Difco™ Novobiocin Supplement, 20mg per vial 231971 6x10 mL


**BBL™ CHROMagar™ Staph aureus is approved by Government of Canada, Health Products and Food Branch, 3rd Supplement, Method MFHPB-21, November 2003.


Reference
1. Escherichia coli O157:H7. 4 Sept 2001. <http:/www.cfsan.fda.gov/~mow/chap15.html>
2. U.S. Food and Drug Administration. 2002. Bacteriological analytical manual (online), Chapter 4A: Diarrheagenic Escherichia coli. AOAC International, Gaithersburg, MD. http://www.cfsan.fda.gov/~ebam/bam-toc.html
3. U.S. Department of Agriculture. 2002. Detection, Isolation and Identification of Escherichia coli O157:H7 and O157:NM (Nonmotile) from Meat Products, Microbiology Laboratory Guidebook MLG 5.03.
4. International Organization for Standards (ISO) Microbiological Methods, ISO 16654: Microbiology of Food and Animal Feeding Stuffs - Horizontal Method for the Detection of Escherichia coli O157, First Edition, 2001-05-01.
5. Data on file, BD Diagnostic Systems, Sparks, MD 21152, USA
6. Wu, V. C. H., Gill, V., Oberst, R., Phebus, R. and Fung, D. Y. C. 2004. "Rapid Protocol (5.25 H) for the Detection of Escherichia Coli O157:H7 in Raw Ground Beef by an Immuno-Capture System (Pathatrix) in Combination with Colortrix and CT-SMAC". J of Rapid Methods and Automation in Microbiology. 12, 57-67.
7. M. A. Joseph, M. Bosilevac, X. Nou, and M. Koohmaraie, Evaluation of Culture- and PCR-Based Detection Methods for Escherichia coli O157:H7 in Inoculated Ground Beef,Terrance U.S. Department of Agriculture, Agricultural Research Service, Roman L. Hruska U.S. Meat Animal Research Center, Clay Center, Nebraska 68933-0166, USA, Journal of Food Protection, Vol68, No. 8, 2005, Pages 1566-1574


AOAC is a trademark and Performance Tested Methods is a service mark of AOAC International. CHROMagar is a trademark of Dr. A. Rambach. PATHATRIX is a trademark of Matrix MicroScience, Inc. Difco is a trademark of Difco Laboratories, Inc., a subsidiary of Becton, Dickinson and Company. BD, BD Logo and BBL are trademarks of Becton, Dickinson and Company. ©2005 BD

Source: BD Diagnostic Systems
Posted: November 11, 2005

March 18, 2010
LATEST NEWS:


Seward Stomacher® 80 microBags for 250µL to 5 mL Volumes

Oxoid Brilliance Staph 24 Agar Identifies Staphylococci in Foods within 24 Hours

New Interscience EasySpiral® for Faster, Easier Spiral Plating

New, Economical, Quality Laboratory Monitoring System

Test for Detection of C. difficile Infection in just 20 Minutes

Environmentally Friendly Bio-bin also Saves Space and Money

New TOS MUP Medium for Enumeration of Bifidobacteria

Lab M's Enterococci Media now Cover Wide Range of Applications

Simple, Rapid PCR for M. tuberculosis Screening

Smooth, Variable Speed Vortex Mixer is Compact and Lightweight

EMLab P&K Provides Salmonella Testing In Light of HVP Scare

Detect More Targets with New Foodproof® GMO Screening Kit, 5'Nuclease!

Hardy Releases Telavancin Susceptibility Disks

New CLSI Report on MRSA Principles, Practices, and Challenges

EMLab P&K Expands Range of PCR Pathogen Tests

New Rapid One Step Assay for Streptomycin in Milk

Quality Management System for Service and Repair of SAS Air Samplers

New Techne TC-PLUS Thermal Cyclers Fast, Flexible and Easy to use

New Sterilin Tubes Make Automated Urinalysis Easy

New AccuPRO-ID™ Microbial Identification Testing Service

New iQ-Check™ High Throughput Protocols, Decreasing Time to Results Even Further.

Ultra High Power LED-520nm for Fluorescence Excitation

Arnie Eyes Up Whitley Workstation at Biofuel Launch

ProtoCOL 2 Enables Rapid, Accurate Colony Counts of Legionella

Lab M Media Selectively Detects E. coli O26 Strain

Now Available - New Anachem RAININ 2010 Catalogue

New BAX® System Test for E. coli O157:H7

Microgen Bioproducts Releases Latest Microlab Newsletter

Microgen Bioproducts Success at Arablab

bioMérieux Launches First Ready-To-Use Enrichment Medium for Campylobacter in Foods

Astell Wins the Space Race

What's Better than a Rayon Swab?- A Medical Wire Rayon Swab!

Compact and Economical Dry Baths

A Material Improvement for the New Generation of Preanalyticals

Improved Identification System for Urogenital Specimens

Bridge4Bio Helps Manufacturers Identify Distribution Channels

MAST Launch Hain GTQ-96 Thermocycler

RAPID'Salmonella Short Protocol Also Detects Non-motile Salmonella

Lab M Spoils its Customers so their Foodstuffs Don't!

New Gilson Distributors for the UK and Ireland

Flocked Swabs Proven Superior in Sample Uptake and Release

Geneius Labs Offer UKAS Accredited rapID® Molecular Identification

AOAC RI Approval for RIDA®COUNT E. coli/Coliform (R1007)

Solve Pipetting Problems for Difficult to Handle Liquids

Beverage Manufacturers get Products to Market Faster and Ensure Safety

New Kits for High Volume Food Pathogen Testing

New Fluorescence and Staining Technologies (F.A.S.T.™) Tuberculosis Products

Sterile Shallow Well Plate Saves Reagents

New ELISA Panel for Gastrointestinal Protozoan Parasites

New Molecular Kit for Endocarditis Testing

New Protocol 2 UV Accessory Counts Fluorescent Colonies and Plaques Fast

Affordable and Flexible Liquid Sample Handling

ATP/AMP Measurement with New Lumitester PD-20!

Easy, Rapid Monitoring of E.coli and Enterococcus spp. in Recreational Water

Oxoid Range Ready for New, Harmonized (EUCAST) European Disc Diffusion Method

Innovative Solution will cut Mycoplasma Detection Time to 4 Hours

New MycAssay™ Pneumocystis Assay for Europe and Canada

New Seward US Offices Achieve Sales Success

AOAC RI for RapidChek® E. coli O157 in Raw Beef Products

 

Top |  Home |  Site Map |  News |  Find Laboratory |  Labs. by Country |  Events
©Copyright rapidmicrobiology.com