| Listeriosis is a foodborne illness caused by L. monocytogenes. It is of particular concern for immunocompromised patients: cancer, HIV, pregnant women, neonates and the elderly. Because of the severity of the disease, 20 deaths per 100 cases, listeriosis is a serious public health and food industry concern. Illness caused by L. monocytogenes has been associated with deli meats, poultry, soft cheeses, ready-to-eat seafood, smoked fish, hot dogs, salad greens and inadequately or unpasteurized milk.[6,7] L. ivanovii, rarely found in foods, is pathogenic to animals and some cases of human listeriosis have been associated with this organism.[8]
BBL CHROMagar Listeria, as a single plate method, is intended for the isolation, differentiation, and confirmatory identification of L. monocytogenes/ivanovii based on the formation of blue-green colonies surrounded by an opaque, white halo in as little as 24 hours from primary enrichment broth. Studies using BBL CHROMagar Listeria for testing a variety of food and environmental samples demonstrated 99-100% sensitivity and 100% specificity with a detection level of 1-18 CFU/25 g.[9]
AVAILABILITY
| Cat. No. | Description | | 215085 | BBL™ CHROMagar™ Listeria, Pkg. of 20 plates | | 290720 | Difco™ Buffered Listeria Enrichment Broth Base, 500 g | | 222330 | Difco™ UVM Modified Listeria Enrichment Broth, 500 g | | 211767 | Difco™ Fraser Broth Base, 500 g | | 211742 | Difco™ Fraser Broth Supplement, 6 x 10 mL | | 218105 | Difco™ Buffered Peptone Water, 500 g |
REFERENCES | 1. | U.S. Food and Drug Administration. 2003. Bacteriological analytical manual (online), AOAC International, Gaithersburg, MD. | | 2. | U.S. Department of Agriculture Food Safety and Inspection Services, Office of Public Health and Science. 2002. Isolation and identification of Listeria monocytogenes from red meat, poultry, egg and environmental Samples. In Microbiology Laboratory Guidebook, Washington, D.C. | | 3. | AOAC Official Methods Listeria monocytogenes in milk and dairy products. 1995. Official Methods of Analysis 16th edition, AOAC International, Arlington, VA. | | 4. | International Organization for Standards (ISO) Microbiology Methods. Microbiology of food and animal feeding stuffs - horizontal method for the detection of Listeria monocytogenes, 1st Edition, 1996 and Amendment 1, 2004, ISO 11290-1 | | 5. | International Organization for Standards (ISO) Microbiology Methods. Milk and milk products - detection of Listeria monocytogenes, 1st Edition, 1993, ISO 1056 0 . | | 6. | Doyle M., L. Beuchat and T. Montville. 2001. Food microbiology fundamentals and frontiers, 2nd ed., American Society for Microbiology, Washington, D.C. | | 7. | Gombas D., Y. Chen, R. Clavero and V. Scott. 2003. Survey of Listeria monocytogenes in ready-to-eat foods, J. Food Prot. 66: 559-569. | | 8. | Reissbrodt, R. 2004. New chromogenic plating media for detection of pathogenic Listeria spp. - an overview, Int. J. Food Microbiol. 95: 1-9. | | 9. | Data on file, BD Diagnostics. |
AOAC is a trademark and Performance Tested Methods is a service mark of AOAC International. ATCC is a trademark of the American Type Culture Collection. CHROMagar is a trademark of Dr. A. Rambach. Difco is a trademark of Difco Laboratories, Inc., subsidiary of Becton, Dickinson and Company. BBL, BD, BD Logo and all other trademarks are the property of Becton, Dickinson and Company © 2005 BD.
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