Once stained, acid acid-fast mycobacteria keep retain their colouring even after treatment with strong decolourizing solutions as HCl-ethanol. They remain red after counterstaining with malachite green, whereas the microorganisms susceptible to acid take on the green.
Merck's Tb-color staining set uses the modified Ziehl-Neelsen staining procedure and eliminates the necessity of heating the carbolfuchsin solution, thus preventing the release of phenol vapour.
As sample materials are suited for use include SputofluolŪ-pretreated smears of sputum, FNAB, lavages, imprints, body fluids, exudates, puss, liquid, and solid cultures. lAll sample materials must be heat-fixed carefully.
The smears can be stained on a staining rack, in coplin jars or in a staining instrument acc. to the protocol. Acid-fast bacteria appear magenta red and the background is light green.
Advantages of this convenient kit include:-
- no heating of carbolfuchsin solution
- effective pretreatment for high-contrast visualization of mycobacteria
- good sensitivity and specificity of the method
|Available products |
|Merck Cat.No |
|Tb-color staining kit 4 x 500 mL |
|Immersion oil 100 mL, 500 mL |
|Immersion oil 100 mL acc. to DIN ISO 8036-1, mod. |
|Oil of cedar wood 100 mL, 500 mL |