| Principle :
RAPID'Listeria spp. has been specifically developed for the detection of all Listeria species in just 24 hrs, after 24 hrs of enrichment, from environmental samples and food. During an inclusivity study, Listeria innocua, L. monocytogenes, L. welshimeri, L. seeligeri, L. ivanovii and L. grayi presented typical blue-green colonies after 24 hrs of incubation.
The chromogenic reaction is based on the specific beta-glucosidase activity of Listeria. The selectivity of the medium inhibits most of the interfering flora and the nutritiousness of the medium provides rapid growth and identification of Listeria spp. in 24 hrs.
Reading and differentiation are very easy thanks to the good contrast between blue-green Listeria colonies and the whitish agar.
Method :
The RAPID'Listeria spp. detection method requires a single enrichment step in ½ Fraser for 24 hrs. Then 0.1 ml of ½ Fraser is removed, spread and isolated on RAPID' Listeria spp. agar, before being incubated for 24 hrs.
Key benefits : - Economical : just 1 broth and 1 plate only are required per sample;
- Rapid response after just 24 hrs of incubation for all Listeria species after enrichment;
- Validated high specificity and selectivity;
- Easy-to to-read chromogenic reaction.
The RAPID'Listeria spp. method is AFNOR-ISO 16140 validated for the detection of Listeria spp. under BRD 07/12 - 12/06. AOAC-RI validation is pending.
For more information, contact your local Bio-Rad representative.
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