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Invitrogen Presents Effective Solutions for Concentration and Isolation of Salmonella Serovars B-Z.

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Recent outbreaks of Salmonella infections in Europe highlights the need for an effective means of isolating Salmonella spp. from food and environmental samples.

Dynabeads® from Invitrogen have long been recognized worldwide as the gold standard magnetic beads for performing immunomagnetic separation.

Dynabeads® anti-Salmonella are designed for rapid selective concentration of Salmonella directly from pre-enriched samples. The product can be used for testing food, water, feed and environmental samples and there is a specific protocol for isolation of Salmonella from eggs.

Dynabeads® anti-Salmonella can either replace or supplement the use of a selective enrichment broth stage.

Methodology
After an 18-24h pre-enrichment in Buffered Peptone Water at 37°C, immunomagnetic separation can be performed either manually, using an MX1 mixer and an MPC-S magnet, or the process can be automated using the Invitrogen BeadRetriever™ instrument. The automated process is fast, easy to use & all the capture and washing steps are totally contained within the instrument. Up to 15 samples can be processed simultaneously with a 1 hour IMS protocol.
Invitrogen BeadRetriever

Following IMS, the Dynabeads® anti-Salmonella and captured target organisms can be plated directly onto standard Salmonella selective plating media. Identification of suspect colonies following incubation can be confirmed using standard biochemical and serological methods.


NOTE: This item is from our 'historic' database and may contain information which is not up to date.

Source: Invitrogen Ltd [USA]
Now part of Life Technologies View company information

Posted: September 18, 2006
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