Loopamp: Nucleic Acid Amplification Kits
Loop Mediated Isothermal Amplification (Loopamp) is a novel gene amplification method using a single enzyme under isothermal reaction conditions. It offers extremely high sensitivity and specificity due to the use of 4 primers recognising 6 distinct regions of the target gene sequence.
The amplification cycle comprises two stages:
2. Isothermal amplification builds upon the dumbell starting unit to form long strands of alternately inverted repeats of the target molecular sequence on the same strand.
Production of huge quantities of nucleic acids (109 -1010 within 15-60 minutes) produces a visibly turbid solution following the Loopamp amplification. This allows the results to be read by eye without the requirement for extra detection steps or expensive equipment. However, some laboratories may wish to use the Loopamp RT Turbidimeter for high throughput use.
Simple procedures, rapid results, increased specificity and minimal laboratory equipment are all advantageous for researchers using nucleic acid amplification techniques.
Loopamp DNA & RNA amplification kits are the ultimate in convenience:
Source: Mast Group Ltd. View latest company information
Posted: July 20, 2009
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