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Listeria - If Cold is not Cold Enough

Listeria colonies

A survivor and beneficiary of modern food

The ability of certain groups of bacteria, and in this case, Listeria spp, to multiply at low temperatures is highly significant from a food hygiene viewpoint. Storing food at low temperatures typically prevents growth of food pathogens, but there is considerable evidence to indicate that this is not the case for Listeria spp.

The Genus Listeria comprises 6 different species, but only L. monocytogenes is generally pathogenic to humans. Listeriosis is an invasive infection caused when contaminated food is ingested. Symptoms vary, ranging from flu-like symptoms to meningitis and encephalitis. The mortality rate for Listeriosis is approximately 23% which, compared to other food poisoning, is very high.
Despite only Listeria monocytogenes being pathogenic to humans, all Listeria species serve as indicators of improper hygiene conditions. It is claimed for example, that a minimum of one food in each fridge is contaminated by Listeria.

Listeria spp. are ubiquitous and a wide variety of food types, such as milk, meat or fish products, can be effected. Not even vegetables can escape being Listeria-free. New technologies, such as cook-chill, and new technologies to extend the shelf life of food could lead to increasing problems with Listeria. Therefore, special care must be taken over the complete food production chain to implement proper hygiene conditions and so prevent contamination with Listeria.

With Merck's comprehensive product range, you are guaranteed to find the product combination which makes the detection of Listeria spp. and Listeria monocytogenes easy, fast and reliable for you.

Regardless of whether you are using the classical media methods, or innovative new methods, such as real time PCR or lateral flow tests, you will guarantee that you are always on the safe side.

Regulation
The following is a summary of Listeria media specified by different regulatory authorities.

    ISO 11290 -1:1996(E)
  • Demi (or Half) FRASER Broth +Supplement
  • FRASER Broth + Supplements
  • AOLA
  • PALCAM
    BAM/FDA
  • Buffered Listeria Enrichment Broth (base) acc. to FDA/BAM 1995
  • PALCAM
  • OXFORD
    FSIS
  • UVM Listeria Selective Enrichment Broth, mod. + Supplements
  • FRASER + Supplement
  • MOX
    IDF Standard 143:1990
  • LEB + Supplement
  • OXFORD Agar


Merck product range for selective enrichment and detection of Listeria spp.

Selective enrichment
Demi (or Half) FRASER Broth

FRASER Broth (base)

FRASER Selective Supplement

FRASER ammonium Iron Supplement

UVM - Listeria Selective Enrichment Broth, mod.

UVM (II) Supplement

Buffered Listeria Enrichment Broth (base)
according to FDA/BAM 1995

Listeria Selective Enrichment Supplement

Listeria Enrichment Broth (LEB) (base)

1.00025.0500

1.138980.500

1.00092.0010

1.00093.0010

1.10824.0500

1.04039.0010

1.09628.0500


1.11781.0010

1.10549.0500
Detection Media
Chromocult® Listeria Selective Agar, (base) (AOLA)
Chromocult® Listeria Agar Selective Supplement
Chromocult® Listeria Agar Enrichment Supplement

PALCAM Listeria Selective Agar, (base)
PALCAM Listeria Selective Supplement

OXFORD Listeria Selective Agar, (base)
OXFORD Listeria Selective Supplement

1.00427.0500
1.00432.0010
1.00439.0010

1.11755.0500
1.12122.0010

1.07004.0500
1.07006.0010
Realtime PCR
TaqMan Instruments and LightCycler 480
foodproof® Listeria monocytogenes Detection Kit
foodproof® Listeria Genus Detection Kit

LightCycler 1.x, 2.0
foodproof® Listeria monocytogenes Detection Kit
foodproof® Listeria Genus Detection Kit

LightCycler 480
foodproof® Listeria monocytogenes Detection Kit
foodproof® Listeria Genus Detection Kit


1.20458.0001
1.20457.0001


1.20452.0001
1.20451.0001



1.20464.0001
1.20463.0001
Confirmation and or Screening
Singlepath® L'mono
Singlepath® Listeria

1.04148.0001
1.04142.0001

For further information about the wide range of products available from Merck Millipore please contact Merck Millipore (see details above) or click the 'More Information' button below.


NOTE: This item is from our 'historic' database and may contain information which is not up to date.

Source: Merck Millipore View latest company information

Posted: December 10, 2010
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